Strain specific CLDN6 expression analysis in heterozygous B-hCLDN6 mice by western blot. Liver tissue was collected from wild-type C57BL/6 (+/+) and heterozygous B-hCLDN6 mice (H/+) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice and heterozygous B-hCLDN6 mice. Human CLDN6 was exclusively detectable in heterozygous B-hCLDN6 mice but not in wild-type mice.

Strain specific CLDN6 expression analysis in homozygous B-hCLDN6 mice by western blot. Liver tissue and thymus were collected from wild-type C57BL/6 (+/+) and homozygous B-hCLDN6 mice (H/H) and analyzed by western blotting with species-specific anti-CLDN6 antibody. Mouse CLDN6 was detectable in wild-type mice. Human CLDN6 was exclusively detectable in homozygous B-hCLDN6 mice but not in wild-type mice.