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B-Rag2/IL2rg KO rats
Strain Name 

SD-Rag2tm1Il2rgtm1/Bcgen

Common Name 

B-Rag2/IL2rg KO rats

Background Sprague Dawley Catalog number 210512
Aliases 

Rag2: RAG-2; 
Il2rg: CD132, [g]c, gamma(c), gc, p64

NCBI Gene ID
295953,140924

B-Rag2/Il2rg KO rats exhibit the smallest thymus size


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The thymus size of B-Rag2/Il2rg KO rats was the smallest compared to that of wild-type SD rats and B-Rag2 KO rats. Gross anatomy of thymuses in male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats were showed (n=3, 7-week-old). Thymus size of B-Rag2 KO rats were significantly reduced compared to that of wild-type SD rats. But the thymus size of B-Rag2/Il2rg KO rats were further reduced compared to B-Rag2 KO rats. 


B-Rag2/Il2rg KO rats exhibit the significantly reduced thymus and spleen weight 


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The size and weight of thymus and spleen of B-Rag2/Il2rg KO rats were significantly reduced compared to that of wild-type SD rats. Thymus and spleen were isolated from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats and weighed (n=3, 7-week-old). Thymus size and weight of B-Rag2 KO rats were significantly reduced compared to that of wild-type SD rats. But the thymus size and weight of B-Rag2/Il2rg KO rats were further reduced compared to B-Rag2 KO rats. Spleen size and weight of B-Rag2 KO rats and B-Rag2/Il2rg KO rats were significantly reduced compared to that of wild-type SD rats. 

Analysis of leukocyte subpopulation in thymus

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Complete loss of T cells in thymus of homozygous B-Rag2 KO rats and B-Rag2/Il2rg KO rats. Thymuses were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that total T cells, CD4+ T cells and CD8+ T cells were only detectable in thymuses of wild-type SD rats. But none of the three types of cells were detectable in thymuses of B-Rag2 KO rats or B-Rag2/Il2rg KO rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in spleen of male rats

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Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from male wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in spleen of female rats

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Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in spleen

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Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in blood of male rats

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Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from male wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in blood of female rats

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Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.

Analysis of leukocyte subpopulation in blood

from clipboard

Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.


MV-4-11 human leukemia xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats


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Subcutaneous homograft tumor growth of MV-4-11 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human biphenotypic B myelomonocytic leukemia cell line MV-4-11 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, MV-4-11 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. B-Rag2/Il2rg KO rats can be used for efficacy studies.  

THP-1 human leukemia xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of THP-1 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human acute monocytic leukemia cell line THP-1 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, THP-1 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.  

MIA PaCa-2 human pancreas cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of MIA PaCa-2 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human pancreas cancer cell line MIA PaCa-2 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, MIA PaCa-2 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.  

NCI-H2122 human lung cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of NCI-H2122 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human non-small cell lung cancer cell line NCI-H2122 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, NCI-H2122 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies. 

H460 human lung cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of H460 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line H460 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, H460 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies. 

NCI-H1373 human lung cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats


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Subcutaneous homograft tumor growth of NCI-H1373 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line NCI-H1373 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, NCI-H1373 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.  

A549 human lung cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of A549 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line A549 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, A549 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.  

RT-112 human bladder cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of RT-112 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human bladder cancer cell line RT-112 (1x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, RT-112 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.  

HCC1954 human breast cancer xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats

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Subcutaneous homograft tumor growth of HCC1954 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human breast cancer cell line HCC1954 (25x106) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, HCC1954 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.