B-Rag2/IL2rg KO rats
| Strain Name |
SD-Rag2tm1Il2rgtm1/Bcgen |
Common Name |
B-Rag2/IL2rg KO rats |
| Background | Sprague Dawley | Catalog number | 210512 |
|
Aliases |
Rag2: RAG-2; |
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NCBI Gene ID |
295953,140924 | ||
B-Rag2/Il2rg KO rats exhibit the smallest thymus size
The thymus size of B-Rag2/Il2rg KO rats was the smallest compared to that of wild-type SD rats and B-Rag2 KO rats. Gross anatomy of thymuses in male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats were showed (n=3, 7-week-old). Thymus size of B-Rag2 KO rats were significantly reduced compared to that of wild-type SD rats. But the thymus size of B-Rag2/Il2rg KO rats were further reduced compared to B-Rag2 KO rats.
B-Rag2/Il2rg KO rats exhibit the significantly reduced thymus and spleen weight
The size and weight of thymus and spleen of B-Rag2/Il2rg KO rats were significantly reduced compared to that of wild-type SD rats. Thymus and spleen were isolated from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats and weighed (n=3, 7-week-old). Thymus size and weight of B-Rag2 KO rats were significantly reduced compared to that of wild-type SD rats. But the thymus size and weight of B-Rag2/Il2rg KO rats were further reduced compared to B-Rag2 KO rats. Spleen size and weight of B-Rag2 KO rats and B-Rag2/Il2rg KO rats were significantly reduced compared to that of wild-type SD rats.
Complete loss of T cells in thymus of homozygous B-Rag2 KO rats and B-Rag2/Il2rg KO rats. Thymuses were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that total T cells, CD4+ T cells and CD8+ T cells were only detectable in thymuses of wild-type SD rats. But none of the three types of cells were detectable in thymuses of B-Rag2 KO rats or B-Rag2/Il2rg KO rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from male wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in spleen of homozygous male B-Rag2/Il2rg KO rats. Spleens were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from male wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
Complete loss of T cells, B cells and NK cells in blood of homozygous male B-Rag2/Il2rg KO rats. Blood cells were collected from male and female wild-type SD rats, B-Rag2 KO rats and B-Rag2/Il2rg KO rats (n=3, 7-week-old). Leukocyte subpopulations were analyzed by flow cytometry analysis. A. Representative FACS plots. B. Statistical analysis of FACS. Results showed that T cells and B cells were not detectable in spleen of B-Rag2 KO rats and B-Rag2/Il2rg KO rats. NK cells were also not detectable in B-Rag2/Il2rg KO rats. But the percentage of NK cells in B-Rag2 KO rats were relatively higher than that in wild-type SD rats. The percentages of DCs, CD11b+CD43+ monocytes, CD11b+CD68+ macrophages, CD11b+CD43+CD68+ monocytes/macrophages (Mo/Mφ) and neutrophils in B-Rag2 KO rats and B-Rag2/Il2rg KO rats were relatively higher than that in wild-type SD rats. Values are expressed as mean ± SEM.
MV-4-11 human leukemia xenograft model in B-Rag2/Il2rg KO rats and B-Rag2 KO rats
Subcutaneous homograft tumor growth of MV-4-11 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human biphenotypic B myelomonocytic leukemia cell line MV-4-11 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, MV-4-11 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. B-Rag2/Il2rg KO rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of THP-1 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human acute monocytic leukemia cell line THP-1 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, THP-1 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of MIA PaCa-2 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human pancreas cancer cell line MIA PaCa-2 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, MIA PaCa-2 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of NCI-H2122 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human non-small cell lung cancer cell line NCI-H2122 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, NCI-H2122 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of H460 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line H460 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, H460 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of NCI-H1373 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line NCI-H1373 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, NCI-H1373 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of A549 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human lung cancer cell line A549 (2x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, A549 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of RT-112 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human bladder cancer cell line RT-112 (1x107) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, RT-112 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
Subcutaneous homograft tumor growth of HCC1954 cells in B-Rag2/Il2rg KO rats and B-Rag2 KO rats. Human breast cancer cell line HCC1954 (25x106) were mixed with Matrigel and inoculated subcutaneously into B-Rag2/Il2rg KO rats and B-Rag2 KO rats (n=5). (A)Tumor volume. (B) Body weight change. As shown in panel A, HCC1954 cells were able to establish tumors in B-Rag2/Il2rg KO rats and B-Rag2 KO rats, and the two strains of rats can be used for efficacy studies.
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