Common name
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B-hCD20-luc GFP A20
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Catalog number
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322285
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Aliases
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/
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Disease
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Reticulum cell sarcoma
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Organism
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Mouse
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Strain
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BALB/cAnN
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Tissue types
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Lymphoma
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Tissue
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B lymphocyte
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Description
The mouse Cd20 gene was replaced by human CD20 coding sequence in B-hCD20-luc GFP A20 cells. Human CD20 is highly expressed on the surface of the cell. And it expresses firefly luciferase and GFP as a marker.
Application
B-hCD20-luc GFP A20 cells can be used for efficacy studies in B lymphoma.
Targeting strategy
Gene targeting strategy for B-hCD20-luc GFP A20 cells. The exogenous promoter, human CD20 coding sequence and luciferase-GFP was inserted to replace part of murine Cd20 gene in B-hCD20-luc GFP A20 cells. Mouse Cd20 transcript was disrupted, the protein no longer be expressed.
Protein expression analysis
CD20 expression analysis in B-hCD20-luc GFP A20 cells by flow cytometry. Single cell suspensions from wild-type A20 and B-hCD20-luc GFP A20 cultures were stained with anti-CD20 antibody. Human CD20 was detected on the surface of B-hCD20-luc GFP A20 cells but not wild-type A20 cells. And mouse CD20 was not detected on B-hCD20-luc GFP A20 cells. And it expresses firefly luciferase and GFP as a marker.
In vitro luciferase activity of B-hCD20-luc GFP A20 cells
Luminescence signal intensity of B-hCD20-luc GFP A20 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-hCD20-luc GFP A20 cells have strong luminescence signal.