B-hMUC16 ID8
|
Common name |
B-hMUC16 ID8 | Catalog number | 321841 |
| Aliases |
CA125 |
Disease | Ovarian Cancer |
|
Organism |
Mouse |
Strain | C57BL/6 |
| Tissue types | Ovary | Tissue | Ovary |
The 13810-14507 aa of human MUC16 coding sequences was inserted to mouse Muc16 gene locus in B-hMUC16 ID8 cells, truncated human MUC16 was highly expressed on the surface of B-hMUC16 ID8 cells. It is recommended to do binding experiments before use.
Targeting strategy
Gene targeting strategy for B-hMUC16 ID8 cells. The exogenous promoter and 13810-14507aa human MUC16 CDS was inserted to mouse Muc16 gene in B-hMUC16 ID8 cells.
MUC16 expression analysis in B-hMUC16 ID8 cells by flow cytometry. Single cell suspensions from B-hMUC16 ID8 cultures were stained with species-specific anti-MUC16 antibody. Human MUC16 were detected on the surface of B-hMUC16 ID8 cells. The 1-F11 clone of B-hMUC16 ID8 cells was used for in vivo experiments.
Subcutaneous homograft tumor growth of B-hMUC16 ID8 cells. B-hMUC16 ID8 cells (5x105) and wild-type ID8 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Body weight (Mean± SEM). (B) Average tumor volume ± SEM. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC16 ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-hMUC16 ID8 tumor cells growth of individual mice. B-hMUC16 ID8 cells (5x105) and wild-type ID8 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6).
（A）On 28 days post inoculation, B-hMUC16 ID8 tumor cells were harvested and assessed for human MUC16 expression by flow cytometry. As shown, human MUC16 was expressed on the surface of tumor cells.
（B）ID8 cells and B-hMUC16 ID8 #1-F11 cells assessed for human MUC16 expression by flow cytometry.
Subcutaneous homograft tumor growth of B-hMUC16 ID8 cells. B-hMUC16 ID8 cells (5x105) and wild-type ID8 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Body weight (Mean± SEM). (B) Average tumor volume ± SEM. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC16 ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-hMUC16 ID8 tumor cells growth of individual mice. B-hMUC16 ID8 cells (5x105) and wild-type ID8 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). As shown in panel, B-hMUC16 ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.
（A）B-hMUC16 ID8 cells were subcutaneously transplanted into C57BL/6 mice, and on 38 days post inoculation, tumor cells were harvested and assessed for human MUC16 expression by flow cytometry. As shown, human MUC16 was expressed on the surface of tumor cells.
（B）ID8 cells and B-hMUC16 ID8 clone 1-F11 cells assessed for human MUC16 expression by flow cytometry.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hMUC16 ID8 cells. B-hMUC16 ID8 cells (5x105, 1x106) and wild-type ID8 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Body weight (Mean± SEM). (B) Average tumor volume ± SEM. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC16 ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.
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