Body weight of B-DIO mice. Male C57BL/6JNifdc mice, aged 5 weeks, were put on a diet consisting of 60 kcal% high-fat feed. Their weights were recorded weekly from a total of 116 mice. The data is presented as Mean ± SD. After being fed with the high-fat diet for 10 weeks, the average weight of the B-DIO mice exceeded 40g.

Glucose tolerance tests of B-DIO mice. Male B-DIO mice were fed a high-fat diet for 12 weeks. After fasting for 16 hours, 10 mice were intraperitoneally injected with 2 g/kg D-Glucose. Blood glucose levels were measured at the following time points after injection: 15 minutes, 30 minutes, 60 minutes, and 120 minutes. The blood glucose levels at 30 minutes, 60 minutes, and 120 minutes of B-DIO mice were significantly higher than those of the control group. Data was shown as Mean ± SD, and analyzed using Multiple t tests. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)

Anti-obesity effect of Dulaglutide in B-DIO mice. (A) Male B-DIO mice were fed a high-fat diet for 12 weeks to induce obesity. Dulaglutide (in house) was administered via subcutaneous injection twice per week for 4 weeks after grouping. (B) Glucose tolerance test were performed the day after the last dose (n=8 mice per group). After treatment with Dulaglutide, both non-fasting and fasting blood glucose levels were reduced compared to those in B-DIO mice. Values are expressed as mean ± SEM.