Strain Name
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C57BL/6-Ms4a1tm2(MS4A1)/Bcgen
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Common Name
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B-hCD20 mice
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Background
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C57BL/6
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Catalog number
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111231
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Related Genes
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CD20, MS4A1,, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1
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NCBI Gene ID
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12482
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mRNA expression analysis
Strain specific analysis of CD20 gene expression in wild type (WT) mice and B-hCD20 mice by RT-PCR.
Mouse CD20 mRNA was detectable only in splenocytes of WT mice (+/+). Human CD20 mRNA was detectable only in homozygous B-hCD20 mice (H/H) but not in WT mice (+/+).
Protein expression analysis in B cells
Strain specific CD20 expression analysis in homozygous B-hCD20 mice by flow cytometry.
Splenocytes were collected from WT and homozygous B-hCD20 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice. Human CD20 was exclusively detectable in homozygous B-hCD20 but not WT mice.