B-hFOLR1/hFOLR2 mice
| Strain Name | C57BL/6-Folr1tm1(FOLR1)Folr2tm1(FOLR2)/Bcgen | Common Name | B-hFOLR1/hFOLR2 mice |
| Background | C57BL/6 | Catalog number | 111065 |
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Related Genes |
FBP; FOLR; NCFTD; Fralpha |
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Strain specific FOLR1 and FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow RT-PCR. Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.
Protein expression analysis
Strain specific FOLR1 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by western blot. Kidney, spleen and brain were collected from C57BL/6 mice (+/+) and homozygous B-hFOLR1/hFOLR2 mice (H/H), and analyzed by western blot with anti-FOLR1 antibody. FOLR1 was detectable in kidney and brain but not spleen from wild-type mice and homozygous B-hFOLR1/hFOLR2 mice, as the antibody is crossly reactive with human and mouse FOLR1.
Strain specific FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow cytometry. Blood was collected from C57BL/6 mice (+/+) and homozygous B-hFOLR1/hFOLR2 mice (H/H), and analyzed by flow cytometry with anti-FOLR2 antibody. hFOLR2 was only detectable in granulocytes, monocytes and macrophages of homozygous B-hFOLR1/hFOLR2 mice but not in wild-type mice.
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