| Strain Name | C57BL/6-Pdcd1tm1(PDCD1)Tnfrsf9tm1(TNFRSF9)/Bcgen | Common Name | B-hPD-1/h4-1BB mice |
| Background | C57BL/6 | Catalog number | 120516 |
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Related Genes |
PD-1 (Programmed death-1) ； TNFRSF9 (TNF receptor superfamily member 9, also known as 4-1BB) |
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NCBI Gene ID |
18566，21942 | ||
Protein expression analysis
Strain specific PD-1 expression analysis in homozygous B-hPD-1/h4-1BB mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/h4-1BB (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 antibody and anti-4-1BB antibody. Mouse PD-1 and 4-1BB were detectable in WT mice. Human PD-1 and 4-1BB were exclusively detectable in homozygous B-hPD-1/h4-1BB but not WT mice.
In vivo efficacy of combination of anti-human PD-1 antibody and anti-human 4-1BB antibody
Antitumor activity of combination of anti-human PD-1 antibody and anti-human 4-1BB in B-hPD-1/h4-1BB mice. (A) combination of anti-human PD-1 antibody and anti-human 4-1BB antibody inhibited MC38 tumor growth in B-hPD-1/h4-1BB mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/h4-1BB mice (female, 6-week-old, n=8). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with anti-human PD-1 antibody and anti-human 4-1BB antibody with doses and schedules indicated in panel. (B) Body weight changes during treatment. As shown in panel A, combination of anti-human PD-1 antibody and anti-human 4-1BB antibody were efficacious in controlling tumor growth in B-hPD-1/h4-1BB mice, demonstrating that the B-hPD-1/h4-1BB mice provide a powerful preclinical model for in vivo evaluation of combination of anti-human PD-1 antibody and anti-human 4-1BB antibody. Values are expressed as mean ± SEM.
