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B-hPD-1/hPD-L1/hTIM3 mice
Strain Name C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Havcr2tm1(HAVCR2)Bcgen/Bcgen
Common Name  B-hPD-1/hPD-L1/hTIM3 mice
Background C57BL/6 Catalog number  130574
Related Genes 
PD-1(Programmed death-1) ;
CD274
(CD274 antigen);HAVCR2 (hepatitis A virus cellular receptor 2)
NCBI Gene ID
18566,60533,171280

Protein expression analysis


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Strain specific PD-1, PD-L1 and TIM3 expression analysis in homozygous B-hPD-1/hPD-L1/hTIM3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hTIM3 (H/H) mice, and analyzed by flow cytometry with species-specific anti-PD-1, anti-PD-L1 and anti-TIM3 antibody. Mouse PD-1, PD-L1 and TIM3 were detectable in WT mice. Human PD-1, PD-L1 and TIM3 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hTIM3 but not WT mice.

Protein expression analysis



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Strain specific PD-1, PD-L1 and TIM3 expression analysis in homozygous B-hPD-1/hPD-L1/hTIM3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hTIM3 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1, anti-PD-L1 and anti-TIM3 antibody. Mouse PD-1, PD-L1 and TIM3 were detectable in WT mice. Human PD-1, PD-L1 and TIM3 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hTIM3 but not WT mice.

Analysis of Blood leukocytes cell subpopulations –No anti-mCD3

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Analysis of Spleen leukocytes cell subpopulations – anti-mCD3


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Analysis of lymph node leukocytes cell subpopulations


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Analysis of blood,spleen and lymph node leukocytes cell subpopulations 


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Analysis of blood,spleen and lymph node leukocytes cell subpopulations by FACS
Blood, spleen and lymph node leukocytes cell were isolated from female mice in the panel(n=3, 6 week-old). Flow cytometry analysis was performed to assess leukocyte subpopulations. Percent of T, B, NK, Granulocytes, Monocyte, DC and macrophage cells in homozygous B-hPD-1/hTIM3, B-hPD-1/hPD-L1/hTIM3 mice were similar to those in the C57BL/6 mice both at rest and in stimulated with anti-CD3ε in vivo, demonstrating that the humanized mouse does not change the overall development, differentiation or distribution of these cell types in blood,spleen and lymph node.

Analysis of blood T cell subpopulations

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Analysis of spleen T cell subpopulations


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Analysis of lymph node T cell subpopulations 


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Analysis of blood,spleen and lymph node T cell subpopulations


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Analysis of blood,spleen and lymph node T cell subpopulations by FACS
Blood, spleen and lymph node leukocytes cell were isolated from female mice in the panel(n=3, 6 week-old). Flow cytometry analysis was performed to assess leukocyte subpopulations. Percent of CD4+T, CD8+T and Treg cells in homozygous B-hPD-1/hTIM3, B-hPD-1/hPD-L1/hTIM3 mice were similar to those in the C57BL/6 mice both at rest and in stimulated with anti-CD3ε in vivo, demonstrating that the humanized mouse does not change the overall development, differentiation or distribution of these cell types in blood,spleen and lymph node.

Combination therapy of anti-human PD-L1 antibody and anti-human TIM3 antibody

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Antitumor activity of anti-human PD-L1 antibody Atezolizumab (in house) combined with anti-human TIM3 antibody hTIM3 Ab (provided by a client) in B-hPD-1/hPD-L1/hTIM3 mice. (A) Anti-human PD-L1 antibody combined with anti-human TIM3 antibody inhibited B-CAG-hPD-L1 MC38 tumor growth in B-hPD-1/hPD-L1/hTIM3 mice. Murine colon cancer B-CAG-hPD-L1 MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hPD-L1/hTIM3 mice (female, 7-8 week-old, n=6). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with atezolizumab (in house) and anti-human TIM3 antibody with doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown in panel A, combination of anti-human PD-L1 antibody and anti-human TIM3 antibody shows more inhibitory effects than individual groups, demonstrating that the B-hPD-1/hPD-L1/hTIM3 mice provide a powerful preclinical model for in vivo evaluating combination therapy efficacy of hPD-L1 antibodies and hTIM3 antibodies. Values are expressed as mean ± SEM.


Combination therapy of anti-human PD-1 antibody and anti-human TIM3 antibody

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Antitumor activity of anti-human PD-1 Keytruda (in house) combined with anti-human TIM3 antibody hTIM3 Ab (provided by a client) in B-hPD-1/hPD-L1/hTIM3 mice. (A) Anti-human PD-1 antibody combined with anti-human TIM3 antibody inhibited B-CAG-hPD-L1 MC38 tumor growth in B-hPD-1/hPD-L1/hTIM3 mice. Murine colon cancer B-CAG-hPD-L1 MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hPD-L1/hTIM3 mice (female, 7-8 week-old, n=6). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with Keytruda (in house) and anti-human TIM3 antibody with doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown in panel A, combination of anti-human PD-1 antibody and anti-human TIM3 antibody shows more inhibitory effects than individual groups, demonstrating that the B-hPD-1/hPD-L1/hTIM3 mice provide a powerful preclinical model for in vivo evaluating combination therapy efficacy of hPD-1 antibodies and hTIM3 antibodies. Values are expressed as mean ± SEM.