top
Please input keywords
Order
*Country
United States
China
France
Germany
Netherlands
United Kingdom
Japan
South Korea
Israel
Australia
Hong Kong, China
New Zealand
Russia
Singapore
Taiwan, China
India
Aland Islands
Albania
Algeria
American Samoa
Andorra
Angola
Anguilla
Antarctica
Antigua & Barbuda
Argentina
Armenia
Aruba
Ascension Island
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belarus
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bosnia & Herzegovina
Botswana
Brazil
British Indian Ocean Territory
British Virgin Islands
Brunei
Bulgaria
Burkina Faso
Burundi
Cambodia
Cameroon
Canada
Canary Islands
Cape Verde
Caribbean Netherlands
Cayman Islands
Central African Republic
Ceuta & Melilla
Chad
Chile
Christmas Island
Cocos (Keeling) Islands
Colombia
Comoros
Congo - Brazzaville
Congo - Kinshasa
Cook Islands
Costa Rica
Côte d’Ivoire
Croatia
Cuba
Curaçao
Cyprus
Czechia
Denmark
Diego Garcia
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
French Guiana
French Polynesia
French Southern Territories
Gabon
Gambia
Georgia
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guam
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Honduras
Hungary
Iceland
Indonesia
Iran
Iraq
Ireland
Isle of Man
Italy
Jamaica
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Kosovo
Kuwait
Kyrgyzstan
Laos
Latvia
Lebanon
Lesotho
Liberia
Libya
Liechtenstein
Lithuania
Luxembourg
Macau, China
Macedonia
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar (Burma)
Namibia
Nauru
Nepal
New Caledonia
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
North Korea
Northern Mariana Islands
Norway
Oman
Pakistan
Palau
Palestinian Territories
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn Islands
Poland
Portugal
Puerto Rico
Qatar
Réunion
Romania
Rwanda
Samoa
San Marino
São Tomé & Príncipe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Sint Maarten
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
South Georgia & South Sandwich Islands
South Sudan
Spain
Sri Lanka
St. Barthélemy
St. Helena
St. Kitts & Nevis
St. Lucia
St. Martin
St. Pierre & Miquelon
St. Vincent & Grenadines
Sudan
Suriname
Svalbard & Jan Mayen
Swaziland
Sweden
Switzerland
Syria
Tajikistan
Tanzania
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad & Tobago
Tristan da Cunha
Tunisia
Turkey
Turkmenistan
Turks & Caicos Islands
Tuvalu
U.S. Outlying Islands
U.S. Virgin Islands
Uganda
Ukraine
United Arab Emirates
United Nations
Uruguay
Uzbekistan
Vanuatu
Vatican City
Venezuela
Vietnam
Wallis & Futuna
Western Sahara
Yemen
Zambia
Zimbabwe
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hTGFBR2/hPDGFRA mice
Strain Name

C57BL/6-Tgfbr2tm3(TGFBR2)Bcgen

Pdgfratm2(PDGFRA)Bcgen/Bcgen

Common Name  B-hTGFBR2/hPDGFRA mice
Background C57BL/6 Catalog number 112766
Aliases 

AAT3, FAA3, LDS2, MFS2, RIIC, LDS1B, LDS2B, TAAD2, 

TBRII, TBR-ii, TGFR-2, TGFbeta-RII; CD140A, PDGFR2, PDGFR-2

NCBI Gene ID
7048, 5156
Description

  • The protein encoded by TGFBR2 is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of genes related to cell proliferation, cell cycle arrest, wound healing, immunosuppression, and tumorigenesis. The PDGFRA encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. 
  • A chimeric CDS that encodes mouse TGFBR2 signal peptide and human extracellular domain, mouse Tgfbr2 transmembrane and cytoplasmic domain, followed by mouse 3’UTR-STOP is inserted right after the exon 2 of mouse Tgfbr2 gene. The chimeric TGFBR2 protein expression will be driven by endogenous mouse Tgfbr2 promoter, while mouse Tgfbr2 gene transcription and translation will be disrupted. The exons 3-11 of mouse Pdgfra gene that encode signal peptide and extracellular domain is replaced by human counterparts in B-hTGFBR2/hPDGFRA mice. The genomic region of mouse Pdgfra gene that encodes cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric PDGFRA expression is driven by endogenous mouse Pdgfra promoter, while mouse Pdgfra gene transcription and translation will be disrupted. . 
  • Human TGFBR2 was exclusively detectable in T, B and NK cells of homozygous B-hTGFBR2/hPDGFRA mice. Human PDGFRA was exclusively detected in kidney, brain and lung of homozygous B-hTGFBR2/hPDGFRA mice. 
  • B-hTGFBR2/hPDGFRA mice provide a powerful preclinical model for in vivo evaluation of anti-TGFBR2 and PDGFRA antibodies.
  • Application: For example, this product is used for pharmacodynamics and safety evaluation of anti-TGFBR2 and PDGFRA antibodies for cancers.

mRNA expression analysis-TGFBR2 and PDGFRA


from clipboard


Strain specific analysis of TGFBR2 and PDGFRA gene expression in wild-type C57BL/6 mice and B-hTGFBR2/hPDGFRA mice by RT-qPCR. Kidney, brain and lung RNA were isolated from wild-type C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H), then cDNA libraries were synthesized by reverse transcription, followed by real-time quantitative PCR with TGFBR2 or PDGFRA primers. A. The mRNA levels of TGFBR2 in homozygous B-hTGFBR2/hPDGFRA mice have a higher level than that of wild-type C57BL/6 mice. B. The kidney mRNA levels of PDGFRA in homozygous B-hTGFBR2/hPDGFRA mice have a higher level than that of wild-type C57BL/6 mice while the lung and brain mRNA levels of PDGFRA in homozygous B-hTGFBR2/hPDGFRA mice were similar to that in C57BL/6 mice.

Protein expression analysis in spleen T cells

from clipboard


Strain specific TGFBR2 expression analysis in homozygous B-hTGFBR2/hPDGFRA mice(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H). Mouse TGFBR2 was detectable in C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-mouse TGFBR2 antibody(R&D, FAB532P), as the antibody is cross-recognized between human and mouse TGFBR2. Human TGFBR2 was exclusively detectable in T cells of homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-human TGFBR2 antibody(Biolegend, 399705).

Protein expression analysis in spleen B cells

from clipboard


Strain specific TGFBR2 expression analysis in homozygous B-hTGFBR2/hPDGFRA mice(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H). Mouse TGFBR2 was detectable in C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-mouse TGFBR2 antibody(R&D, FAB532P), as the antibody is cross-recognized between human and mouse TGFBR2. Human TGFBR2 was exclusively detectable in B cells of homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-human TGFBR2 antibody(Biolegend, 399705).

Protein expression analysis in spleen NK cells

from clipboard


Strain specific TGFBR2 expression analysis in homozygous B-hTGFBR2/hPDGFRA mice(H/H) by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H). Mouse TGFBR2 was detectable in C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-mouse TGFBR2 antibody(R&D, FAB532P), as the antibody is cross-recognized between human and mouse TGFBR2. Human TGFBR2 was exclusively detectable in NK cells of homozygous B-hTGFBR2/hPDGFRA mice(H/H) using anti-human TGFBR2 antibody(Biolegend, 399705).

Protein expression analysis

from clipboard


Strain specific PDGFRA expression analysis in homozygous B-hTGFBR2/hPDGFRA mice by Western Blot. Kidney, brain and lung tissues were were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hTGFBR2/hPDGFRA mice(H/H), and then analyzed by western blot with anti-mouse PDFGRA antibody (R&D, AF1062-SP) and anti-human PDFGRA antibody (R&D, AF-307-SP). Mouse PDGFRA was exclusively detected in kidney, brain and lung of wild-type mice. Human PDGFRA was exclusively detected in kidney, brain and lung of homozygous B-hTGFBR2/hPDGFRA mice. 

Frequency of leukocyte subpopulations in spleen

from clipboard


Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice and homozygous B-hTGFBR2/hPDGFRA mice (n=3, 7-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, neutrophils, CD4+ T cells, CD8+ T cells and tregs in B-hTGFBR2/hPDGFRA mice were similar to those in C57BL/6 mice, demonstrating that humanization of TGFBR2 and PDGFRA does not change the frequency or distribution of these cell types in spleen. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001.