| Strain Name |
C57BL/6-Fcgrttm1(FCGRT)Bcgen/Bcgen
|
Common Name | B-hFcRn mice |
| Background | C57BL/6 | Catalog number |
110001 |
|
Related Genes |
FCGRT(Fc fragment of IgG receptor and transporter) |
||
|
NCBI Gene ID |
14132 | ||
mRNA expression analysis
Strain specific analysis of FCGRT gene expression in WT and B-hFcRn mice by RT-PCR.
Mouse Fcgrt mRNA was detectable in kidney of wild-type (+/+). Human FCGRT mRNA was detectable only in H/H but not in +/+ mice.
Protein expression analysis
Strain specific analysis of FcRn expression in C57BL/6(+/+) and B-hFcRn mice by western blot.
Kidney was collected from C57BL/6(+/+) mice and homozygous B-hFcRn(H/H) mice and analyzed by western blot with species-specific anti-FcRn antibody. Mouse FcRn was detectable in C57BL/6(+/+) mice. Human FcRn was detectable only in B-hFcRn(H/H) but not in C57BL/6(+/+) mice.
Serum half-life of Ab1 in B-hFcRn mice
PK profiles in B-hFcRn and C57BL/6 mice IV-dosed at 10 mg/kg with Ab1. Data points from mice with high immune response to the injected antibody, demonstrated by mouse antihuman antibody titers of greater than 1 to 1000 and corresponding to abrupt PK changes, were excluded in the analyses.
Different type of IgGs pharmacokinetics in B-hFcRn mice
PK profiles in B-hFcRn and C57BL/6 mice IV-dosed at 10 mg/kg with Ab1(IgG1), Ab2(IgG2) and Ab3(IgG3). Data points from mice with high immune response to the injected antibody, demonstrated by mouse antihuman antibody titers of greater than 1 to 1000 and corresponding to abrupt PK changes, were excluded in the analyses.
The YTE mutation prolongs Abs half-life in B-hFcRn mice
- The test drugs are commercial drugs
- The half-life in humans and monkeys of test drugs from research articles
- Blood collection: Continuous
- Abrupt PK changes were excluded in the analyses
- Curve fitting: The last three blood collection points
- Data are shown in mean ± SEM
