Gene targeting strategy for B-Cd155-EGFP KI mice. The EGFP gene encoding green fluorescent protein was inserted after the 5 'UTR sequences of mouse Cd155 gene to replace it in B-Cd155-EGFP mice.

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen，bone marrow，lymph node and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was not detectable in B cells of wild-type mice and homozygous B-Cd155-EGFP KI mice. EGFP was detectable in B cells of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, lymph node, thymus and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in T cells of wild-type mice, but not detectable in T cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in T cells of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, thymus, bone marrow and lymph node were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in NK cells of wild-type mice, but not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Blood was collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in macrophages of wild-type mice, but not detectable in macrophages of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in macrophages of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in monocytes of wild-type mice, but not detectable in monocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in monocytes of homozygous B-Cd155-EGFP KI mice. 

Strain specific mCD155 and EGFP expression analysis in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, blood and bone marrow were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 antibody and anti-EGFP antibodies. Mouse CD155 was detectable in granulocytes of wild-type mice, but not detectable in granulocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in granulocytes of homozygous B-Cd155-EGFP KI mice.