| Strain Name |
|
Stock No. | 110161 |
| Common name |
B-IL17a-EGFP mice |
Gene symbol and name | IL17a(interleukin 17A), Ctl; IL-17 |
| Strain of origin |
C57BL/6 |
Chromosome | 1 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
16171 | ||
Application
- Function research of genes
- This model is an efficient tool in tracking the expression pattern of IL17A with EGFP fluorescence.
Expression
Expressed gene
EGFP, enhanced green fluorescent protein
mouse Grp is highly expressed both in the lateral nucleus of the amygdala, the nucleus
Site of expression
Fluorescence can be observed in some intraepithelial lymphocytes (IELs) of the small intestine without stimulation
Gene editing strategy
The Il17aEGFP mice were developed by Beijing Biocytogen Co., Ltd. An IRES-EGFP-SV40-polyA signal sequence cassette was inserted after the stop codon of the Il17a gene using C57BL/6-derived embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Fig1. Compared with wild type mice,IL17-GFP
mouse express enhanced green fluorescent protein (EGFP) as a marker of IL17A
activity.
Fluorescence can be observed in some
intraepithelial lymphocytes (IELs) of the small intestine without stimulation.
Naive CD4+ T cells stimulated with plate-bound anti-CD3/anti-CD28 in the
presence of transforming growth factor, beta 1 (TGFB, 1 ng/ml) and interleukin
6 (IL6, 20 ng/ml) show increased fluorescence associated with IL17A production.
Normal levels of IL17A are expressed.
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alpha signaling constrains the development of IL-17-producing gammadelta T
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