|Disease||Biphenotypic B myelomonocytic leukemia|
This B-Luc-EGFP MV-4-11 cell line expresses firefly luciferase as a marker of MV-4-11 cells. Luminescence could be detected in B-Luc-EGFP MV-4-11 cells.
B-Luc-EGFP MV-4-11 cell line can be used to evaluate anti-cancer drugs in biphenotypic B myelomonocytic leukemia in vivo.
Gene targeting strategy for B-Luc-EGFP MV-4-11 cells. The exogenous promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.
In vitro luciferase activity of B-Luc-EGFP MV-4-11 cells
Luminescence signal intensity of B-Luc-EGFP MV-4-11 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc-EGFP MV-4-11 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP MV-4-11 cells. The 1-D02 clone of B-Luc-EGFP MV-4-11 cells can be used in vivo experiments.
Quantitative imaging data & body weight changes