|Lewis lung carcinoma
This B-Luc LLC1 cell line expresses firefly luciferase as a marker of LLC1 cells. Luminescence can be observed in B-Luc LLC1 cells.
Luminescence signal intensity of B-Luc LLC1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc LLC1 cells have a strong luminescence signal. The 1-H04 clone of B-Luc LLC1 cells can be used in vivo experiments.
Subcutaneous homograft tumor growth of B-Luc LLC1 cells. B-Luc LLC1 cells (1x105 and 2x105) and wild-type LLC1 cells (1x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Luc LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.
Quantitative imaging data & body weight changes
In vivo luciferase images of B-Luc LLC1 (C57BL/6, B-Luc LLC1, 1x105, s.c.)