Description 

This B-Luc LLC1 cell line expresses firefly luciferase as a marker of LLC1 cells. Luminescence can be observed in B-Luc LLC1 cells.

Application
B-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia B-Luc LLC1 cell line can be used to evaluate anti-cancer drugs in Lewis lung carcinoma in vivo. Targeting strategy
Gene targeting strategy for B-Luc LLC1 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.

 

In vitro luciferase activity of B-Luc LLC1 cells

Luminescence signal intensity of B-Luc LLC1 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc LLC1 cells have a strong luminescence signal. The 1-H04 clone of B-Luc LLC1 cells can be used in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-Luc LLC1 cells. B-Luc LLC1 cells (1x10⁵ and 2x10⁵) and wild-type LLC1 cells (1x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.

Quantitative imaging data & body weight changes

Imaging analysis in vivo

In vivo luciferase images of B-Luc LLC1 (C57BL/6, B-Luc LLC1, 1x105, s.c.)