||B-hCD19 EL4||Catalog number||310712|
|Aliases||CD19, CD19 molecule, B4, CVID3||Disease||Lymphoma|
|Tissue types||Lymphoma||Tissue||T lymphocyte|
The mouse Cd19 gene was replaced by human CD19 coding sequence in B-hCD19 EL4 cells. Human CD19 is highly expressed on the surface of B-hCD19 EL4 cells.
B-hCD19 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Gene targeting strategy for B-hCD19 EL4 cells.
The exogenous promoter and human CD19 coding sequence was inserted to replace part of murine exons 2-12. The insertion disrupts the endogenous murine CD19 gene, resulting in a non-functional transcript.
Protein expression analysis
The CD19 expression analysis in B-hCD19 EL4 cells by flow cytometry. Single cells were collected from wild-type EL4 and B-hCD19 EL4 cells, and analyzed by flow cytometry with species-specific anti-CD19 antibody. Human CD19 was detectable in B-hCD19 EL4 cells but not wild-type EL4 cells. The 2-D07 clone of B-hCD19 EL4 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes