|MS4A1, Bp35, CVID5
The human CD20 coding sequence was inserted into the ROSA26 locus in MC38 cells. Human CD20 is highly expressed on the surface of B-hCD20 MC38 cells.
Gene targeting strategy for B-hCD20 MC38 cells. The exogenous promoter and human CD20 coding sequence was inserted into the ROSA26 locus in murine.
CD20 expression analysis in B-hCD20 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD20 MC38 cultures were stained with species-specific anti-CD20 antibody. Human CD20 was detected on the surface of B-hCD20 MC38 cells but not wild-type MC38 cells. The 2-A12 clone of B-hCD20 MC38 cells was used for in vivo experiments.