Common name
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B-hGUCY2C MC38
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Catalog number
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321837
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Aliases
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DIAR6, GC-C, GUC2C, MECIL, MUCIL, STAR
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Disease
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Colon carcinoma
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Organism
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Mouse
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Strain
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C57BL/6
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Tissue types
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Colon
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Tissue
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Colon
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Description
The chimeric CDS consist of human GUCY2C extracellular plus mouse Gucy2c intracellular region sequences was inserted to mouse Gucy2c gene exon 1 in B-hGUCY2C MC38 cells, so that the mouse GUCY2C was not expressed anymore, human GUCY2C was highly expressed on the surface of B-hGUCY2C MC38 cells.
Application
B-hGUCY2C MC38 cells have the capability to establish tumors in C57BL/6 mice in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-hGUCY2C MC38 cells. The exogenous promoter and chimeric CDS consist of human GUCY2C extracellular plus mouse Gucy2c intracellular region sequences was inserted to mouse Gucy2c gene exon 1 in B-hGUCY2C MC38 cells.
Protein expression analysis
GUCY2C expression analysis in B-hGUCY2C MC38 cells by flow cytometry. Single cell suspensions from B-hGUCY2C MC38 cultures were stained with species-specific anti-GUCY2C antibody. Human GUCY2C were detected on the surface of B-hGUCY2C MC38 cells. The 2-B03 clone of B-hGUCY2C MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hGUCY2C MC38 cells. B-hGUCY2C MC38 cells (1x106 ,5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hGUCY2C MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Protein expression analysis of tumor cells
B-hGUCY2C MC38 cells were subcutaneously transplanted into C57BL/6N mice (n=8). At the end of the experiment, tumor cells were harvested and assessed for human GUCY2C expression by flow cytometry. As shown, human GUCY2C was highly expressed on the surface of tumor cells. Therefore, B-hGUCY2C MC38 cells can be used for in vivo efficacy studies of novel GUCY2C therapeutics.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hGUCY2C MC38 cells. B-hGUCY2C MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice mice (female, 9-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hGUCY2C MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
