This B-hPD-L1 Raji-luc cell line expresses firefly luciferase as a marker of Raji cells. Fluorescence and luminescence can be observed in B-hPD-L1 Raji-luc cells.
B-hPD-L1 Raji-luc cell line can be used to evaluate anti-cancer drugs in lymphoma in vivo.
Protein expression analysis
PD-L1 expression analysis in B-hPD-L1 Raji-luc cells by flow cytometry. Single cell suspensions from B-luc-GFP Raji and B-hPD-L1 Raji-luc cultures were stained with species-specific anti-PD-L1 antibody. Human PD-L1 was detected on the surface of B-hPD-L1 Raji-luc cells but not B-luc-GFP Raji cells. The 1-H11 clone of B-hPD-L1 Raji-luc cells was used for in vivo experiments.
In vitro luciferase activity of B-hPD-L1 Raji-luc cells
Tumor growth and in vivo imaging of B-hPD-L1 Raji-luc cells. B-luc-GFP Raji cells and B-hPD-L1 Raji-luc cells (5x105) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-hPD-L1 Raji-luc cells can be used for in vivo efficacy evaluation.