Description 
The overexpression of OVA in B16-F10-OVA tumor cells can improve the immunogenicity of tumor cells and be used for screening the efficacy of immuno-oncology (I/O) therapeutics.

Application

B16-F10-OVA cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis

OVA expression analysis in B16-F10-OVA cells by flow cytometry. Single cell suspensions from wild-type B16-F10 and B16-F10-OVA cultures were stained with species-specific anti-OVA antibody. OVA was detected on the surface of B16-F10-OVA cells but not wild-type B16-F10 cells. The clones 1-G12 and 1-G04 of B16-F10-OVA cells were used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B16-F10-OVA cells. B16-F10-OVA cells (2x10⁵) and wild-type B16-F10 cells (2x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B16-F10-OVA cells were able to establish tumors in vivo and can be used for efficacy studies.