|Gene symbol and name||Oxt (oxytocin), OT, Ox, Oxy|
|Strain of origin||
|Coat color||White||Dietary information||Growth and reproduction diet for experimental mice|
NCBI Gene ID
iCre, improved cre recombinase, bacteriophage P1
Site of expression
supraoptic nucleus, paraventricular nuclei, the magnocellular subnuclei of PVN
Gene editing strategy
A P2A-iCre sequence cassette was inserted upstream 3’UTR of the Oxt gene. This strain was maintained on a Sprague Dawley genetic background.
(A) Schematic of AAV-Retro-DIO-YFP viral injection in OXT-Cre rats.
(B) Immunostaining of YFP with OXT-NP on viral injected OXT-Cre rat brain slices. Co-localization of YFP with OXT-NP is indicated with white triangles.
(C) Parvocellular neurons in the PVN were negative for YFP.
(J) Schematic injection and chemogenetic inhibition of Magno-OXT neurons with AAV-Retro-DIO-hM4Di.
(K) Representative images showing the viral infection of Magno-OXT neurons. Co-localization of mCherry with OXT-NP is indicated with white triangles.
(source: Zhang B et al.,2021)
1. Zhang B, Qiu L, Xiao W, Ni H, Chen L, Wang F, Mai W, Wu J, Bao A, Hu H, Gong H, Duan S, Li A, Gao Z. Reconstruction of the Hypothalamo-Neurohypophysial System and Functional Dissection of Magnocellular Oxytocin Neurons in the Brain. Neuron. 2021 Jan 20;109(2):331-346.e7. [PubMed: 33212012]2. Armstrong WE, Warach S, Hatton GI, McNeill TH. Subnuclei in the rat hypothalamic paraventricular nucleus: a cytoarchitectural, horseradish peroxidase and immunocytochemical analysis. Neuroscience. 1980;5(11):1931-58. [PubMed: 7432630]