|Gene symbol and name||Tagln, transgelin|
|Strain of origin||
|Coat color||Black||Dietary information||Growth and reproduction diet for experimental mice|
NCBI Gene ID
iCre, improved cre recombinase, bacteriophage P1
Site of expression
smooth muscle cell
Gene editing strategy
ICRE, WPRE and PolyA cassette were inserted into the first intron of mouse endogenous transgelin gene to express iCre recombinase regulated by the Tagln promoter of mouse endogenous gene, and the PolyA element knocked out the Tagln gene in mice.The correct genotype mice were obtained by microinjection of mouse ES cells. This design is based on transcript-201 (ENSMUST00000034590.3, NM_011526 ) This gene encodes a smooth muscle cell-specific cytoskeletal protein. The encoded protein is structurally similar to calponin, an actin-binding protein. In mouse models of atherosclerosis the gene product may be involved in plaque cell and atherosclerotic lesion formation during atherogenesis.
The frozen sections of the stomach (a), aorta (b), jejunum (c) and bladder (d) of mice were detected by IF, respectively, and the results showed that tdTomato specific expression in Tagln-iCre (Mut /+);CAG-tdTomato (Mut / +) genotypes of double positive mice in alpha SMA markers of smooth muscle cells (with SMA protein markers of smooth muscle cells, the vast majority of the proportion of cells containing tdTomato red fluorescent protein signals, but it is not 100%, analyze the possible reasons may be the efficiency of the recombinant enzyme wasn't 100%), can attest, iCre recombinant enzyme specificity expressed in smooth muscle cells, other tissue cells tdTomato red fluorescent protein signal wasn’t detected. The mice in the negative control group also did not express tdTomato red fluorescent protein.
1.Yang M, Jiang H, Li L. Sm22α transcription occurs at the early onset of the cardiovascular system and the intron 1 is dispensable for its transcription in smooth muscle cells during mouse development. Int J Physiol Pathophysiol Pharmacol. 2010;2(1):12-9. Epub 2009 Nov 22. [PMID: 20428474]; [PMCID: PMC2860299]
2.Zhang JC, Kim S, Helmke BP, Yu WW, Du KL, Lu MM, Strobeck M, Yu Q, Parmacek MS. Analysis of SM22alpha-deficient mice reveals unanticipated insights into smooth muscle cell differentiation and function. Mol Cell Biol. 2001 Feb;21(4):1336-44. [PMID: 11158319]; [PMCID: PMC99586]
3.Lepore JJ, Cheng L, Min Lu M, Mericko PA, Morrisey EE, Parmacek MS. High-efficiency somatic mutagenesis in smooth muscle cells and cardiac myocytes in SM22alpha-Cre transgenic mice. Genesis. 2005 Apr;41(4):179-84. [PMID: 15789423]
4.Regan CP, Manabe I, Owens GK. Development of a smooth muscle-targeted cre recombinase mouse reveals novel insights regarding smooth muscle myosin heavy chain promoter regulation. Circ Res. 2000 Sep 1;87(5):363-9. [PMID: 10969033]
5.Yan J, Zhang L, Sultana N, Park DS, Shekhar A, Bu L, Hu J, Razzaque S, Cai CL. A Murine Myh6MerCreMer Knock-In Allele Specifically Mediates Temporal Genetic Deletion in Cardiomyocytes after Tamoxifen Induction. PLoS One. 2015 Jul 23;10(7):e0133472. [PMID: 26204265]; [PMCID: PMC4512710]