Multiple sclerosis (MS) model
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system that leads to encephalitis and demyelination. MS is considered an autoimmune disease caused by autoreactive T cells with symptoms including muscle rigidity and paralysis, visual disturbance and blindness, sensory loss and ataxia, and is characterized by recurrent relapses. At present, there are many different animal models of MS, of which the experimental autoimmune encephalomyelitis (EAE) model is widely used in the study of multiple sclerosis due to its pathological characteristics of inflammation and demyelination similar to MS.
In rodents such as mice and rats, EAE models can be induced by immunization with spinal cord homogenates, purified myelin, myelin proteins (e.g., myelin basic protein MBP, protein lipoprotein PLP, and myelin oligodendrocyte glycoprotein MOG), or peptides of these proteins. This may be because myelin-specific T cells are activated in the periphery, cross the blood-brain barrier into the central nervous system and are reactivated, triggering a series of inflammatory responses, leading to demyelination and axonal cell apoptosis, ultimately leading to nerve injury and loss of function. Clinical symptoms are assessed using a standardized scoring system, which measures the degree of induction of the disease, with focal demyelination and inflammatory leukocyte infiltration seen in the stained sections of the pathological tissue.
The MOG-induced EAE disease model protocol described here was established in C57BL/6 mice and in B-hIL17A humanized mice developed by Biocytogen, andcan be used for pharmacodynamic evaluation of MS-related drugs.