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Introduction of Nonalcoholic Steatohepatitis (NASH) Model

Nonalcoholic fatty liver disease (NAFLD) is a disease in which fat accumulates excessively in the liver. This fat accumulation is not caused by heavy alcohol consumption. Non-alcoholic steatohepatitis (NASH) is one type of NAFLD. In addition to liver fat accumulation, there are pathological changes of hepatitis, hepatocyte injury, fibrosis and liver scar formation, which may lead to cirrhosis or liver cancer. The mechanism of NASH progression remains unclear, and effective treatments are still lacking.

The clinical symptoms of NASH are quite complex and include obesity, insulin resistance, steatohepatitis, hepatocyte ballooning, and fibrosis according to the disease process. NASH models often used in preclinical experiments are genetic animal models, diet-induced animal models, and animal models constructed by combining genetics with diet-inductioned. However, it is difficult to mimic all the pathogenetic features of human diseases in a short period of time.

To understand the pathogenic mechanisms underlying the development and progression of NASH and to develop innovative therapies, we developed several mouse models of different stages of NASH pathogenesis.

  • Western diet (WD): Dietary components include high fructose and high cholesterol, and this diet-induced NASH model develops obesity, impaired glucose tolerance and hepatic steatosis.
  • High-fat methionine-choline-deficient diet (HFMCD): The diet contains 60 kcal% fat, and is deficient in methionine and choline. , and this diet-induced NASH model shows increased liver injury, hepatic steatosis, and fibrosis accompanied by increased NAS scores.
  • STAM-NASH: The Stelic Animal Model (STAM) mice are the first animal model of NASH-related liver carcinogenesis resembling disease development in humans.
NASH Mouse Model Induced by Western Diet(WD)
High-fat methionine-choline-deficient diet (HFMCD) -induction of NASH in mice
STAM mouse model of NASH Model