Inflammatory bowel disease (IBD) refers tochronic inflammatory disease of the colon or gastrointestinal tract, including ulcerative colitis (UC) and Crohn's disease (CD) Clinical symptoms of IBD include recurrent diarrhea, abdominal pain, intestinal bleeding/hematochezia, fever, and weight loss. The pathogenesis of IBD is not clear, and studies have shown that various factors such as genes, immune system, external environment and intestinal microorganisms are associated with the occurrence of disease. A variety of preclinical mouse models are currently used to study IBD, and disease models derived based on different induction methods have specific uses. The mouse enteritis model induced by dextran sulfate sodium (DSS) is the most widely used chemically-induced mouse IBD model. Acute ulcerative enteritis or chronic colitis isinduced by dissolving DSS in drinking water, which destroys intestinal epithelial cells in mice, releases cytokines by non-specific immune cells, and finally results in disruption of the integrity of the mucosal barrier. Animals experience significant weight loss, loose stools, hematochezia, and granulocyte infiltration, symptoms that are extremely similar to human ulcerative colitis in clinical symptoms and pathological features.
A stable DSS-induced IBD disease model protocol was established inC57BL/6 mice by Biocytogen, which can be used for preclinical studies and pharmacodynamic evaluation of inflammatory enteritis.